Upon tamoxifen injection into
Here, we describe protocols for optimal DNA recombination in astrocytes, based on the GLAST-Cre ERT2 /loxP system
The Cre/lox site-specific recombination system allows the control of gene activity in space and time in almost any tissue of the mouse
Our aim was to
(A) Tamoxifen (Tam)-inducible System of estrogen receptor fused to Cre (CreER)
Here we report that tamoxifen injection in young Rosa26-CreERT2 (R26CreERT2) pups (P9 to P11) in the absence of a floxed allele results in severe
In this study we focused on tamoxifen degradation kinetics, because for all genetic fate-mapping studies, the period during The Cre-Lox system enables recombination of a pair of short (34 bp) DNA sequences called Lox sequences by the recombinase encoded by the bacteriophage P1 gene Cre
2
2 mg applied to newborn mouse pups in 10 μl Miglyol vehicle was adequate to successfully drive Cre recombinase-mediated genome rearrangements by the fifth day of life, in a murine model of BPD
The injected tamoxifen is metabolized in the liver to 4-hydroxytamoxifen, 4-OHT
J
A major tamoxifen administration is performed by intraperitoneal administration or oral administration
The Cre/lox site-specific recombination system has emerged as an important tool for the generation of conditional somatic mouse mutants
Add 100 Tamoxifen is commonly used as a cancer treatment in humans and for inducing genetic alterations using Cre-lox mouse models in the research setting
The Cre/loxP method allows for the genetic tagging of one or several cells in a temporally inducible manner in order to perform lineage analysis in mouse embryos and adults ()
This powerful tool greatly facilitates the study
Tamoxifen (TAM)-inducible Cre recombinase is widely used for tissue-specific and temporal induction of gene deletion in mice
However, the expression of Cre recombinase in a tissue-specific manner often produces undesirable changes in mouse biology that can confound data interpretation when using these tools to generate