In the time-course
This study aimed to determine the effect of dexamethasone (Dex) on the immunomodulatory function of iPSC-MSCs in vitro and in vivo
Our findings demonstrate that a synchronized circadian rhythm in cell culture based in vitro test systems can significantly increase the sensitivity towards the cellular
Dexamethasone exposure reduced the total number of T cells in the majority of T cell subsets along the differentiation spectrum in the tumor-draining lymph nodes
Research by Hislop et al
The effect of dexamethasone on morphogenesis and differentiation of cells obtained from mouse embryos grown at high density in vitro was investigated
The effect of dexamethasone, cyclosporine, and rapamycin on T-lymphocyte proliferation in vitro: Comparison of cells from C2C12 cells were seeded in culture medium 24 h before measurement at a density of 3 × 10 4 cells per well, and then treated with DMSO or 1 μM Dex for 6 h for analysis
Based on the results of the present research, we concluded that the addition of dexamethasone partially counteracts the repressive effect of TNF-α on follicle growth and E 2 secretion during in vitro culture of the preantral follicles of mice
When studying nuclear morphology using Hoechst 33342, increased apoptosis was seen already at 1 µM dexamethasone and the percentage of apoptotic cells increased in a dose-dependent manner with higher dexamethasone concentrations ( Figure 3A,B )
In in vitro culture systems, dexamethasone (DEX) has been applied with ascorbic acid (ASC) and β-glycerophosphate (βGLY) as culture media supplementation to induce osteogenic differentiation of mesenchymal stem cells
The study of the circadian clock and its mechanisms is easily facilitated through clock resetting in cell culture
After co-culture, non-engulfed target cells were washed away extensively with phosphate-buffered saline, and C2C12 cells were detached by trypsinization at 37 °C for 15 min and filtered through a The rates of spontaneous cell detachment, cell viability, and apoptosis in primary cultures of rat hepatocytes plated at high and low density were compared
In this study, we evaluate the combination activity of rapamycin and dexamethasone and characterize a molecular mechanism of synergy between those agents using a larger panel of in vitro and in vivo models of lymphoid malignancies
Cell lines from human T-cell leukemia established from children at diagnosis (COG-LL-329h) or at relapse (COG-LL-317h 11 systemic xenograft model, consistent with the high sensitivity of the cell line in vitro to dexamethasone, the EFS was extended in mice treated with dexamethasone (P < 0
S1 G) Our findings demonstrate that a synchronized circadian rhythm in cell culture based in vitro test systems can significantly increase the sensitivity towards the cellular responses upon treatment with chemicals
After 3 weeks in culture, cells were fixed and stained with Alizarin red